Figure 2.

Effect of FPP, Lys, and Leu on fatigue-related biochemical parameters in muscle after treadmill stress test. After the last treadmill exercise, muscle samples were obtained from each mouse. (a) Lactate, (b) LDH, (c) glycogen, and (d) CK were measured with each kit. (e) L6 cells were treated with FPP, Lys, and Leu for 24 h except FBS. The viability of L6 cells was measured by a MTT assay. Values are the mean ± SEM. n = 5 per group. CK: creatine kinase, FBS: fetal bovine serum, FPP: fermented porcine placenta, LDH: lactate dehydrogenase, Lys: lysine, Leu: leucine, MTT: 3 -(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, SEM: standard error mean. *P < 0.05, significantly different from the control mice