Figure 2.

(a,b) The expression of the PKIB mRNA and protein was examined by real-time PCR and western blotting, respectively. The results showed that the expression of the PKIB mRNA and protein was significantly increased in the PKIB-transfected cells transfected compared with the vector group. (c) Overexpression of PKIB increased the viability of the A549 cells, as determined by the MTT assay. (d) The results of the BrdU assay implied that BrdU incorporation was enhanced by PKIB overexpression. (e) PKIB overexpression induced PCNA expression in A549 cells. All values are denoted as the means ± SEM from three or more independent batches of cells (*P < 0.05)