Figure 3.

Real-time PCR (a) and western blotting (b) were applied to confirm the efficiency of the PKIB shRNA infection in H1299 cells. PKIB shRNA significantly decreased the expression of the PKIB mRNA and protein. (c,d) Cell viability (c) and BrdU incorporation (d) were decreased after the expression of PKIB was knocked down with PKIB shRNA. (e) PCNA expression was down-regulated in H1299 cells after PKIB inhibition. “NC” means negative control, and all values are denoted as the means ± S.E.M. from at least three separate experiments (n = 3, *P < 0.05)