Enzyme reaction mechanisms of BCO2. BCO2, which is located on the inner membrane of mitochondria, catalyzes carotenoids by cleaving double bond at position 9, 10 and 9′, 10′ of carotenoids. BCO2, β,β-carotene-9′, 10′-oxygenase 2. (A color version of this figure is available in the online journal.)