Table 3.
Associations between methylation frequencies detected by the two methods and clinical pathology
| Characteristics | N | AuNPs
|
qMSP
|
||||
|---|---|---|---|---|---|---|---|
| M | Percentage (%) | P-value | M | Percentage (%) | P-value | ||
| Age (n=42) | 0.828 | 0.506 | |||||
| Mean (SD) (range) | 47 (10.5) (20–74) | ||||||
| <30 | 6 | 2 | 33.33 | 3 | 50.00 | ||
| 30–50 | 24 | 10 | 41.67 | 14 | 58.33 | ||
| >50 | 12 | 6 | 50.00 | 9 | 75.00 | ||
| Cytology results | 0.389 | 0.305 | |||||
| SCC | 2 | 2 | 100.00 | 2 | 100.00 | ||
| HSIL | 10 | 5 | 50.00 | 8 | 80.00 | ||
| LSIL | 7 | 4 | 57.14 | 5 | 71.43 | ||
| ASCUS-H | 8 | 3 | 37.50 | 5 | 62.50 | ||
| ASCUS | 12 | 3 | 25.00 | 5 | 41.67 | ||
| Normal | 3 | 1 | 33.33 | 1 | 33.33 | ||
| Pathology results | <0.001 | 0.006 | |||||
| SCC | 3 | 3 | 100.00 | 3 | 100.00 | ||
| CIN3 | 7 | 7 | 100.00 | 7 | 100.00 | ||
| CIN2 | 7 | 4 | 57.14 | 6 | 85.71 | ||
| CIN1 | 13 | 2 | 15.38 | 6 | 46.15 | ||
| Normal | 12 | 2 | 16.67 | 4 | 33.33 | ||
| HPV | 0.344 | 0.236 | |||||
| Positive | 35 | 16 | 45.71 | 23 | 65.71 | ||
| Negative | 7 | 2 | 28.57 | 3 | 42.86 | ||
Notes: P-value was obtained using chi-square test. P<0.05 was considered statistically significant difference.
Abbreviations: ASCUS, atypical squamous cells of undetermined significance; ASCUS-H, high-grade atypical squamous cells of undetermined significance; AuNPs, gold nanoparticles; CIN1, cervical intraepithelial neoplasia type 1; CIN2, cervical intraepithelial neoplasia type 2; CIN3, cervical intraepithelial neoplasia type 3; HSIL, high-grade squamous intraepithelial lesion; HPV, human papillomavirus; LSIL, low-grade squamous intraepithelial lesion; M, methylated number; qMSP, quantitative methylation-specific polymerase chain reaction; SCC, squamous cell carcinoma; SD, standard deviation.