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Product accumulation and electron distribution for wild-type MoFe protein
| Gas | Product
|
|||||||
|---|---|---|---|---|---|---|---|---|
| H2
|
HCOO−
|
CH4
|
CO
|
|||||
| nmol/nmol/mina | % e− | nmol/nmol/min | % e− | nmol/nmol/min | % e− | nmol/nmol/min | % e− | |
|
|
||||||||
| Ar | 83 ± 1.55 | 100 | NDb | ND | ND | ND | ND | ND |
| CO2 | 70 ± 1.8 | 87 | 9.8 ± 1.5 | 12 | ND | ND | 0.03 | 1 |
nmol product/nmol MoFe protein/min. Rates are the average over the 60 min assay at 30°C with 1 mg MoFe protein and 1 mg Fe protein. CO2 was at a partial pressure of 0.45 atm.
ND, not detected.
