Fig. (10).

Compounds 13, 25, and 14 inhibit palmitoylated Ras-dependent growth in yeast. A representative (n=3) spot assay of cytotoxic effects in wild type (RJY1941) S. cerevisiae is compared to that of the palmitoylation-dependent (RJY1942) strain. Liquid cultures containing the shown inhibitors were spotted onto selective media and incubated at 30°C for 3 days. The concentrations used in the liquid cultures for compounds 13, 25, 14 and 2-BP were 2.5 μM, 5 μM, 10 μM, and 20 μM in 1% DMF.