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. 2016 May;19(4):262–274. doi: 10.2174/1386207319666160324123844

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© 2016 Bentham Science Publishers

This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) ( https://creativecommons.org/licenses/by-nc/4.0/legalcode ), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Fig. (6) — Inhibition of Erf2 auto-palmitoylation measured by a gel-based assay. An orthogonal screen of steady-state Erf2 auto-palmitoylation with 100 μM of each of the ten individual compounds (43, 27, 19, 13, 22, 34, 14, 25, 30, and 28) was separated by SDS-PAGE. The average relative BODIPY^® fluorescence from three reactions that co-migrated with Erf2 is presented as a fraction of vehicle control (V; 5% DMF) +/- standard deviation. A reaction lacking Erf2 (-) represents baseline activity in the assay. 100 μM 2-BP is a control for inhibition of Erf2 steady-state auto-palmitoylation.