Fig. (7).

Michaelis-Menten plots for Compounds 13 and 25, and 2-BP. The velocity of Erf2 auto-palmitoylation as altered by the addition of the inhibitors at 100 μM (empty diamonds), 50 μM (filled squares), 25 μM (empty triangles), and vehicle control (1% DMF; filled circles) for compounds 13 and 25, and 2-BP. The velocity of Erf2 auto-palmitoylation was detected as an increase in fluorescence over time. V_MAX is the maximum velocity of the reaction, and K_M is the substrate concentration that allows the reaction to reach a velocity that is 50% of the V_MAX. On the Michaelis-Menton Plot, a competitive inhibitor would increase K_M without altering V_MAX, whereas an uncompetitive inhibitor would result in a decrease of V_MAX without altering the K_M of the reaction.