Fig. (8).

Inhibition of palmitoyl-CoA concentration dependent palmitoylation of Erf2 by Compound 13. Orthogonal screen of steady-state Erf2 auto-palmitoylation with compound 13 at 25 μM (dark grey bars), 50 μM (light grey bars) and 100 μM (white bars) was separated by SDS-PAGE. The average relative BODIPY^® fluorescence from three reactions that co-migrated with Erf2 is presented as a fraction of vehicle control (VC; 5% DMF) for each concentration of BODIPY^®-C12-CoA +/- standard deviation. A reaction lacking Erf2 (-) represents baseline activity in the assay. Prior experiments in this study used 100 μM Compound 13 with 40 μM BODIPY^®-C12-CoA.