FIG 9.

Saffold virus infection affects the development of the Purkinje cell. On day 22 p.i., the original AM or mouse-passaged AM-5Cb strain intracerebrally inoculated (10⁴ CCID₅₀s/10 μl) neonatal ddY mice were used for high-resolution fluorescence imaging. (A) Three-dimensional images of sections of the cerebellar layer at a depth of 50 μm that had been immunofluorescence stained with anti-calbindin antibody. The stack images were acquired with a 0.46-μm step size in the z direction. y-z and x-z images are shown in the sagittal slice of a stack image. (B) The width of the Purkinje cell layer and the molecular layer of the stack images at a 50-μm depth were determined with Neurolucida software. Data are from four areas per mouse. (C) A representative tracing image of the soma and main dendrites of a Purkinje cell. (D) Soma number and area in Purkinje cells were measured by using Neurolucida software. Data are from four areas per mouse. (E) Average diameter and length of the main dendrites of Purkinje cells. An average of 35 cells were measured in four areas of the cerebellum per mouse. The main dendrites of AM-5Cb-inoculated mice are significantly shorter than those of control mice. *, P < 0.05; one-way ANOVA. Original magnification, ×300 (A, C).