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. 2016 Oct 14;90(21):10007–10021. doi: 10.1128/JVI.00864-16

TABLE 1.

Primer and probe sequences used for real-time RT-PCR

Test, primers, and probe Genome sequence (5′–3′)
Real-time RT-PCR for Saffold virus
    Forward AAACCATGCCACAAACACCAT
    Reverse GCCYTGACCAACTACCCACAT
    Probe (FAM-TAMRA) CTTGCCGAYACACGTGACCCACA
Real-time RT-PCR for murine Hes5
    Forward CCTGAAACACAGCAAAGCCTTC
    Reverse GGAGTAGCCCTCGCTGTAGTC
    Probe (FAM-TAMRA) CCGGCCCCAAGAGCCTGCACC
Real-time RT-PCR for murine DNER
    Forward TGTCCTAGACCCATGCAGAAATG
    Reverse CAAGCGGAGCCGAAGTACC
    Probe (FAM-TAMRA) TGAACCCGCTGAGGCTGGACACACA
Real-time RT-PCR for murine GLAST
    Forward GATGCTGCAGATGCTGGTCTT
    Reverse TTACTATCTAGGGCCGCCATTC
    Probe (FAM-TAMRA) CCCCTGATCATCTCCAGTCTCGTCACA
Real-time RT-PCR for murine Calbindin
    Forward GCTCCGCGCACTCTCAA
    Reverse GAGATGACTGCAGGTGGGATTC
    Probe (FAM-TAMRA) AGCCGCTGCACCACGATGGC