FIG 2.

Isolated viral factories contain the core protein L410. (A) Western blots of total cell lysates at different postinfection time points were incubated with anti-core protein (L410) antibodies. L410 was detected only at later stages of infection, matching our mass spectroscopy findings. (B) Western blots of purified viral factories were incubated with anti-L410 antibodies. L410 was detected in viral factories, as well as in the virion, only at 7 h p.i., thus further substantiating our MS results. (C) Immunolabeling of cryopreserved cells at 8 h p.i. with rabbit anti-L410 antibodies, followed by exposure to gold-conjugated secondary antibody, revealed that the core protein is present in the viral factory as well as in the assembling virions. Scale bar: 1 μm.