FIG 3.

Eukaryotic translation initiation factor 4E-like protein L496 and ribosomes are not incorporated into the viral factories. (A) Western blots of total cell lysates at different postinfection time points were incubated with anti-4E-like protein (L496) antibodies. L496 was detected only in the total cell lysates and not in the factories, as indeed indicated by our MS findings. (B) TEM of intracellular replication centers at 4 h p.i. in the process of coalescing into a single viral factory. The micrograph reveals ribosomes (dark dots with diameters of ∼25 to ∼30 nm) that surround the replication centers but are not incorporated into these organelles. Rc and m, replication centers and mitochondria, respectively. Asterisks represent the cores of the original infecting virus (26). Scale bar: 500 nm.