Skip to main content
. 2016 Oct 14;90(21):9632–9643. doi: 10.1128/JVI.01353-16

FIG 2.

FIG 2

Expression of apoE in uninfected cells is important for their permissiveness to HCV infection. (A) Western blot verification of apoE expression in Huh7.5.1 and derived sh-NT, sh-apoE, apoE2HA, apoE3HA, and apoE4HA cell lines; tubulin was detected as a loading control. Proteins are specified on the right. Positions of molecular mass standards are indicated on the left. (B to F) Detection of infectivity of sh-NT virus (B), sh-apoE virus (C), apoE3HA virus (D), HCVTCP (E), and lenti-GFP virus (F) was determined by using a limiting-dilution assay in 96-well plates with Huh7.5.1, sh-NT, sh-apoE, apoE2HA, apoE3HA, and apoE4HA cells, respectively. After 72 h of culture, cells were fixed and probed by anti-NS5A antibody to specify infection-positive wells. Shown are the means from 3 independent experiments; error bars indicate standard deviations from the means.