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. 2016 Oct 14;90(21):9632–9643. doi: 10.1128/JVI.01353-16

FIG 7.

FIG 7

apoE exchange between lipoprotein particles produced from Huh7.5.1 cells. (A) Western blot verification of apoE3HA and apoE3Flag expression in Huh7.5.1 cells. Tubulin was detected as a loading control. Proteins are specified on the right. (B) Density profile of secreted apoE3HA and apoE3Flag. Cell culture supernatants containing apoE3HA and apoE3Flag were collected, concentrated by ultrafiltration, and fractionated by density gradient centrifugation. Fifteen fractions were recovered from each gradient and analyzed by Western blotting using antibodies against HA tag and Flag tag. (C) Equal volumes of apoE3HA medium and apoE3Flag medium were mixed and incubated for 4 h at 37°C. HA or Flag affinity purification of lipoprotein particles and Western analysis were conducted to test apoE exchange between lipoprotein particles from apoE3HA- and apoE3Flag-expressing cells. Proteins are specified on the right. Positions of molecular mass standards are indicated on the left.