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. 2016 Oct 14;90(21):9664–9673. doi: 10.1128/JVI.01224-16

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Copyright © 2016 Lu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Expression of EBV proteins Zta and Rta has no effect on the efficiency of ICP0-null mutant HSV-1 infection. (A) HepaRG-based cells constructed to express FLAG-tagged Zta or myc-tagged Rta were analyzed by immunofluorescence before or after induction with doxycycline, as indicated. Pairs of images in vertical alignment show the EGFPnlsTetR fusion protein and the relevant epitope tag signals from the same field of cells. The controls are HA-TetR parental cells. (B) The same cells were analyzed by Western blotting for expression of FLAG-tagged Zta and myc-tagged Rta before and after induction. (C) ICP0-null mutant plaque formation was analyzed in induced control and Zta- and Rta-expressing cells. Dox, doxycycline.