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. 2016 Oct 14;90(21):9664–9673. doi: 10.1128/JVI.01224-16

FIG 4.

FIG 4

The effects of expression of BNRF1, EBNA-LP, and EBNA1 on ICP0-null mutant HSV-1 gene expression in HFT cells. (A) Western blot analysis of induced expression of EBNA-LP or BNRF1 or the combination of both in HFT-based cells. (B) Inducible expression of EBNA1 in HFT-derived cells, which were used for the control. (C) The efficiency of ICP0-null mutant HSV-1 plaque formation was analyzed in cells analogous to the above after treatment with doxycycline, using the approach described in the legends of Fig. 1 to 3. The results are expressed as fold increases above the level in control HFT cells. (D) Western blot analysis of cells constructed to express combinations of HCMV proteins IE1 and pp71 or of IE1 with BNRF1. (E) Plaque formation analysis on ICP0-null mutant HSV-1 in HFT-based cells expressing combinations of IE1 and pp71 or of IE1 and BNRF1 in comparison with control HFT cells.