Skip to main content
. 2016 Oct 14;90(21):9664–9673. doi: 10.1128/JVI.01224-16

FIG 6.

FIG 6

BNRF1 increases viral gene expression during pp71 mutant HCMV infection. (A) Control HFT cells expressing EYFP or FLAG-tagged BNRF1 were treated with doxycycline and then infected with AD169sub82 (MOI of 1). Samples were prepared over the following days and analyzed for expression of BNRF1 and HCMV proteins IE1, IE2, UL44, and pp28. (B) BNRF1 increases viral gene expression from pp71 mutant HCMV almost as efficiently as pp71. Cells induced to express myc-tagged pp71 or FLAG-tagged BNRF1 were infected with AD169sub82 (MOI of 1), and then samples were prepared over the following days and analyzed for expression of BNRF1 and HCMV proteins IE1, IE2, UL44, and pp28. (C) Plaque formation analysis of pp71-null mutant HCMV strain AD169sub82 in cells induced to express pp71 or BNRF1.