FIG 10.

Disrupting PI3K and p21-activated kinase 1 (Pak1) signaling blocks HCMV infection. HS-578T cells were pretreated with the Pak1 inhibitor IPA-3 at16.7 μM (A) or at the indicated concentration (B) or treated with the PI3K inhibitor LY294002 (12.5 μM) (E) and infected with HCMV as described for Fig. 7. Infection was monitored either by RT-qPCR for HCMV UL123 at 5.5 h postinoculation (A) or by FACS for HCMV-GFP positive cells at day 3 postinfection (E). The level of GAPDH RNA (C) and cell viability (D) were measured to assess potential cytotoxicity associated with IPA-3 as described in the legend to Fig. 7.