FIG 3.
FISH of intravitreally injected eyes 3 days postinjection. (a) PBS-injected eyes served as a negative control for FISH detection and had low background. The arrowheads indicate FISH signal. Weak signal was detected in the retinas of eyes injected with 1 × 108 vg for rAAV2 and rAAV2-R585E. rAAV2 at a dose of 5 × 108vg showed accumulation of the transgene at the ILM (arrows), with some detectable transgenes in the outer retina (arrowheads). A dose of 5 × 108 vg led to weak signal for rAAV2-R585E. A high dose of 2 × 109 vg revealed greater accumulation of rAAV2 capsids at the ILM (arrows) and within the retina (arrowheads). At the same dose, rAAV2-R585E showed a greater presence in the retina within the ONL (arrowheads). (b) Schematic of the retina depicting the trafficking of rAAV following IVit delivery. Scale bar = 20 μm.