FIG 2.

Functional testing of CCR7/CD62L transductants. (A) The induction of MAPK/ERK pathway within CCR7/CD62L T-cell transductants (R7/62L) in response to the CCR7 ligand (CCL21)is presented. (Top) ERK1/2 and phospho-ERK immunoblots of cell lysates are presented, with the minutes of CCL21 exposure denoted above each sample, molecular mass standards indicated at left, and bands identified at right. (Bottom) A graph of the measured band intensities is presented with minutes of CCL21 exposure denoted under each sample. (B) A micrograph of CCL21-induced cell migration assayed by transwell CCR7-mediated chemotaxis is presented above a graphic summary of cell counts measured in the transwell assay. Data are representative of results of two independent experiments. (C) Upper row: a flow cytometric analysis for CD62L and IFN-γ of rhesus PBMC left unstimulated or stimulated with anti-CD3 is presented. Lower group: results of flow cytometric analysis of R7/62L cells for CD62L and IFN-γ are presented. Data are representative of results of two independent experiments.