FIG 2.

Binding profiles of P[19] VP8* with human milk and porcine mucin and comparison of saliva binding profiles between P[10] and P[19]VP8* proteins. The human milk and porcine mucin samples were fractioned through a Superdex 200 column before testing for binding to P[19] VP8* by following a procedure described previously (24, 25). Binding signals peaked in the high-molecular-weight fractions for both human milk (a) and porcine mucin (b) samples. The P[19] VP8* also revealed a binding profile similar to that of another RV (P[10] in P[I] [d]) among 96 adult human saliva samples tested (c). The ABH and Lewis epitope statuses of the saliva donors are indicated. Each column represents the mean A₄₅₀, and the error bars represent the standard deviation for each sample tested in triplicate.