FIG 9.

Binding of P[II] RV VP8*s to mucin cores 2, 4, and 6 and type 1 HBGA-related glycans. (a) Binding of the P[4], P[6], and P[8] VP8* proteins to mucin cores 2, 4, and 6. Two doses (2 and 20 μg/ml) of the mucin cores were tested. Positive binding signals were observed for P[4] and P[8] but not for P[6]. (b) Binding of the P[II] RVs to the type 1 precursor disaccharide lacto-N-biose (Galβ1-3GlcNAc). Low binding signals were detected for all four P[II] RVs. (c to e) Binding of the P[II] RV VP8* proteins with the type 1 HBGAs LNT, LNFPI, and LNDFPI. The P[6] and P[19] VP8* proteins showed positive binding to the type 1 tetrasaccharide LNT (Galβ1-3GlcNAcβ1-3Galβ1-4Glc) (c) and the LNFPI pentasaccharide (Fucα1-2 Galβ1-3GlcNAcβ1-3Galβ1-4Glc) (d) with and without the terminal fucose modification (secretor), respectively. Neither of the P[4] and P[8] VP8* proteins bound the LNT and LNFPI oligosaccharides. (e) Adding a Lewis fucose to LNFPI results in the LNDFHI hexasaccharide (Fucα1-2 Galβ1-3(Fucα1-4)GlcNAcβ1-3Galβ1-4Glc), which led to negative P[6] and P[19] VP8* binding but positive P[4] and P[8] VP8* binding. The mean A₄₅₀s from three replicates with standard deviations are shown.