Extended data Figure 3. The interscale is maintained by two cell populations during homeostasis.

(a) Evolution in time of the total labelled cell fraction under three hypotheses. For a perfect single population of equipotent balanced progenitors, the labelled cell fraction remains constant. For a single population of equipotent balanced progenitors displaying short-term priming, the labelled cell fraction increases transiently for the cells primed to divide, and decreases transiently for the cells primed to differentiate, but after the priming period, both fractions remain constant at different values. For two populations organised in a hierarchy, the labelled fraction of the progenitors decreases continuously to zero, while the labelled fraction of the stem cells continuous increases to reach a steady state value, corresponding to its average progeny size. (b) Cumulative basal clone size distribution of Inv-CreER/Rosa-YFP clones at homeostasis in the interscale upon Tamoxifen administration. (c) Cumulative basal clone size distribution of K14-CreER/Rosa-YFP clones at homeostasis in the interscale upon Tamoxifen administration. Clonal distributions are plotted in log-plot, error bars indicate S.D., thick lines are the model prediction and shaded area indicate 95% confidence intervals in the model prediction.