Table 2. Effects of chronic ethanol intake on the Bdnf gene methylation in the dentate gyrus, CA1 and CA3 subfields of the hippocampus.
| Bdnf CpG Islands | Water | Ethanol |
|---|---|---|
| Dentate gyrus | ||
| I | 1.000±0.006 (6) | 1.006±0.010 (8) |
| II | 1.000±0.009 (6) | 1.001±0.007 (8) |
| III | 1.000±0.012 (6) | 0.987±0.006 (8) |
| IV | 1.000±0.010 (6) | 1.006±0.005 (8) |
| V | 1.000±0.010 (6) | 1.004±0.007 (8) |
| VI | 1.000±0.009 (6) | 1.002±0.006 (8) |
| VII | 1.000±0.008 (6) | 1.014±0.003 (8) |
| Gapdh | 1.000±0.008 (6) | 1.011±0.006 (8) |
| Bdnf CpG Islands | Water | Ethanol* |
|---|---|---|
| CA1 | ||
| I | 1.000±0.013 (5) | 0.976±0.006 (8) |
| II | 1.000±0.014 (5) | 0.969±0.006 (8) |
| III | 1.000±0.014 (5) | 0.977±0.004 (8) |
| IV | 1.000±0.012 (5) | 0.974±0.009 (8) |
| V | 1.000±0.013 (5) | 0.956±0.008 (8) |
| VI | 1.000±0.009 (5) | 0.985±0.008 (8) |
| VII | 1.000±0.015 (5) | 0.985±0.014 (8) |
| Gapdh | 1.000±0.014 (5) | 0.991±0.011 (8) |
| Bdnf CpG Islands | Water | Ethanol** |
|---|---|---|
| CA3 | ||
| I | 1.000±0.009 (6) | 0.991±0.008 (8) |
| II | 1.000±0.010 (6) | 0.985±0.008 (8) |
| III | 1.000±0.005 (6) | 0.997±0.009 (8) |
| IV | 1.000±0.005 (6) | 0.998±0.007 (8) |
| V | 1.000±0.007 (6) | 0.988±0.008 (8) |
| VI | 1.000±0.010 (6) | 0.984±0.008 (8) |
| VII | 1.000±0.006 (6) | 0.982±0.008 (8) |
| Gapdh | 1.000±0.006 (6) | 0.997±0.007 (8) |
*P<0.05.
**P<0.0001.
Methylation enrichment levels on the different Bdnf CpG islands were measured by real-time quantitative PCR of the immunoprecipitated DNA with a specific antibody against 5-methylcytidine in the three subfields of hippocampus in ‘water' and ‘ethanol' mice. Results (mean±s.e.m. of (n) mice) are expressed as arbitrary units after normalization of the immunoprecipitated DNA/input DNA ratio to that of the control group of ‘water' mice for each CpG island. A two-way analysis of variance showed an effect of ethanol on the methylation of Bdnf CpG islands in CA1 and CA3 subfields.