Table 1. Macrostomum species and specimens used in our study.
| Culture | Year | Collection sites | Culture conditions | Reference |
|---|---|---|---|---|
| (a) Inbred lines and outbred cultures of M. lignano | ||||
| Inbred lines | ||||
| DV1 | 2003 | Bibione (site UV, N45.63405, E13.07626), Italy | 32‰ f/2 in glass Petri dishes | [14] |
| HUB1 | A transgenic line created from DV1 | 32‰ f/2 in glass Petri dishes | [15] | |
| Outbred cultures | ||||
| LS1 | 2003 | Bibione (site UV) and Isola di Martignano (site PS, N45.70383, E13.15793), Italy | 32‰ f/2 in glass Petri dishes | [17] |
| LS2 | 2011 | Bibione (close to site UV) and Lignano Sabbiadoro (close to site P1, N45.6918, E13.1312), Italy | 32‰ f/2 in glass Petri dishes | this study |
| LS3 | 2013 | Vourvourou (N40.2029, E23.7672) and Porto Koufo (N39.9595, E23.9278), Sithonia Peninsula, Greece | 32‰ f/2 in glass Petri dishes | this study |
| IBK2 | 2012 | Lignano Sabbiadoro (close to site P1), Italy collected by Peter Ladurner, Uni Innsbruck | 32‰ f/2 in glass Petri dishes | this study |
| (b) Freshly field-collected specimens of M. lignano and Macrostomum sp. 8 | ||||
| M. lignano | 2014 | Lignano Sabbiadoro (close to site P1) | 32‰ ASW* in PS culture plates | this study |
| Macrostomum sp. 8 | 2014 | Palavas-les-Flots (N43.4994, E3.8694) | 32‰ ASW in PS culture plates | this study |
| (c) Cultures of other Macrostomum species | ||||
| M. spirale (outbred culture) | 2004 | Étang de Biguglia (N42.6591, E9.4504) | 6‰ ASW in glass Petri dishes | this study |
| M. hystrix (inbred line SR1) | 2010 | San Rossore Regional Park (N43.6843, E10.2830), Pisa, Italy | 6‰ ASW in glass Petri dishes | [6] |
(a) inbred lines and outbred cultures of Macrostomum lignano
(b) freshly field-collected specimens of M. lignano and Macrostomum sp. 8, and
(c) cultures of other Macrostomum species (M. spirale and M. hystrix) used in our study, listing the year of establishment/collection, the site of collection, the laboratory culture conditions, and the reference if already published.
Sites UV, PS, and P1 are described in [1], although note that there were some errors in the reported coordinates for some collection sites.
ASW*–artificial sea water