Table 2. Morphometric analysis of Macrostomum karyotypes.
| AL (μm) | RL (%) | L (μm) | S (μm) | R | CI | |
|---|---|---|---|---|---|---|
| (a) Macrostomum lignano | ||||||
| 1 | 5.27 ± 0.81 | 41.24 ± 1.5 | 2.81 ± 0.46 | 2.46 ± 0.39 | 1.15 ± 0.13 | 0.47 ± 0.03 (m) |
| 2 | 2.74 ± 0.27 | 21.54 ± 0.63 | 1.47 ± 0.16 | 1.26 ± 0.16 | 1.18 ± 0.16 | 0.46 ± 0.03 (m) |
| 3 | 2.49 ± 0.25 | 19.59 ± 0.56 | 1.34 ± 0.15 | 1.15 ± 0.13 | 1.17 ± 0.11 | 0.46 ± 0.02 (m) |
| 4 | 2.24 ± 0.29 | 17.62 ± 0.01 | 1.19 ± 0.14 | 1.06 ± 0.19 | 1.13 ± 0.12 | 0.47 ± 0.07 (m) |
| (b) Macrostomum sp. 8 | ||||||
| 1 | 6.34 ± 0.35 | 32.28 ± 0.61% | 3.29 ± 0.13 | 3.05 ± 0.21 | 1.06 ± 0.06 | 0.43 ± 0.06 (m) |
| 2 | 5.89 ± 0.39 | 31.62 ± 1.08% | 2.89 ± 0.19 | 2.86 ± 0.21 | 1.07 ± 0.09 | 0.47 ± 0.01 (m) |
| 3 | 2.98 ± 0.02 | 14.16 ± 0.01% | 1.55 ± 0.01 | 1.43 ± 0.03 | 1.11 ± 0.01 | 0.47 ± 0.01 (m) |
| 4 | 2.83 ± 0.09 | 13.54 ± 0.25% | 1.59 ± 0.09 | 1.24 ± 0.19 | 1.37 ± 0.19 | 0.42 ± 0.02 (m) |
| 5 | 2.36 ± 0.02 | 11.76 ± 0.86% | 1.23 ± 0.06 | 1.13 ± 0.04 | 1.08 ± 0.02 | 0.45 ± 0.02 (m) |
| (c) Macrostomum spirale | ||||||
| 1 | 3.75 ± 0.47 | 36.72 ± 1.35 | 2.06 ± 0.35 | 1.67 ± 0.20 | 1.22 ± 0.18 | 0.45 ± 0.03 (m) |
| 2 | 3.37 ± 0.34 | 33.11 ± 0.91 | 1.87 ± 0.26 | 1.57 ± 0.28 | 1.22 ± 0.12 | 0.45 ± 0.02 (m) |
| 3 | 3.07 ± 0.28 | 30.16 ± 1.13 | 1.67 ± 0.17 | 1.45 ± 0.27 | 1.20 ± 0.12 | 0.45 ± 0.02 (m) |
| (d) Macrostomum hystrix | ||||||
| 1 | 3.76 ± 0.54 | 38.07 ± 1.34 | 2.09 ± 0.37 | 1.78 ± 0.32 | 1.26 ± 0.37 | 0.46 ± 0.03 (m) |
| 2 | 3.31 ± 0.48 | 32.67 ± 1.19 | 2.1 ± 0.35 | 1.27 ± 0.27 | 1.85 ± 0.34 | 0.37 ± 0.06 (sm) |
| 3 | 2.96 ± 0.45 | 29.26 ± 1.08 | 1.94 ± 0.36 | 1.04 ± 0.18 | 1.90 ± 0.30 | 0.35 ± 0.04 (sm) |
(a) Macrostomum lignano based on 'normal' 2n = 8 metaphase plates (N = 50)
(b) its close relative Macrostomum sp. 8 based on 'normal' 2n = 10 metaphase plates (N = 35), and two more distantly related species, namely
(c) M. spirale 2n = 6 (N = 20) and
(d) M. hystrix 2n = 6 (N = 20).
The reported values represent means±1SD and include the absolute length of each chromosome (AL), the relative length of each chromosome, the length of the long arm (L), the short arm (S) and the arm ratio (R = L/S); and the centromeric index [CI = S/(L+S)] (m, sm stand for metacentric and submetacentric, correspondingly).