Table 1. Antibodies information.
Conjugated primary Ab | Company/Cat# | Application | Dilution |
PE-Mouse-CXCR-4 | BD Cat# 561733 | FC* | 1:20 |
APC-Mouse-c-Kit | Thermo Fisher Cat# CD11705 | FC | 1:20 |
PE-Mouse-NKX6.1 | BD Cat# 563023 | FC | 1:20 |
PE-Mouse-IgG2a isotype | BD Cat# 551438 | FC | 1:20 |
Unconjugated primary Ab | Company/Cat# | Application | Dilution |
Rabbit FOXA2 | Abcam Cat# ab40874 | IF** | 1:200 |
Goat SOX17 | R&D Cat# AF1924 | IF | 1:200 |
Guinea Pig PDX1 | Abcam Cat# ab47308 | FC/IF | 1:10000 |
Goat NGN3 | Santa Cruz Cat # sc-13793 | FC/IF | 1:50 |
Rabbit NGN3 | Abcam Cat# ab38548 | FC/IF | 1:100 |
Mouse C-peptide | Millipore Cat# 05–1109 | FC/IF | 1:100 |
Guinea Pig Insulin | Dako Cat# A0564 | FC/IF | 1:200 |
Mouse NKX6.1 | Hybridoma Bank Cat# F55A12 | IF | 1:50 |
Rabbit MAFA | Custom Ab, Lifespan Biosciences, Seattle | IF | 1:200 |
Rabbit Glucagon | Cell signaling Cat# D16G10 | FC/IF | 1:100 |
Rabbit Somatostatin | Thermo Fisher Cat# PA1-30636 | FC/IF | 1:100 |
Mouse NeuroD1 | Abcam Cat# ab60704 | IF | 1:100 |
Mouse Syntaxin-1A | Thermo Fisher Cat# MA5-17612 | IF | 1:200 |
Rabbit Synaptophysin | Cell Signaling Cat# D35E4 | IF | 1:200 |
Rabbit ARX | Abcam Cat# ab111063 | IF | 1:25 |
Mouse PAX4 | Hybridoma Bank Cat# M-Pax4-1F3A3 | IF | 1:25 |
* FC: Flow Cytometry
**IF: Immunofluorescent staining