Table 1. Sample size of each experimental condition and the sequence of different experiments in male offspring.
Experiments | n6-SAL | n6-POL | n3-SAL | n3-POL | Age (days) | |
---|---|---|---|---|---|---|
Neuroimaging | MRS | 10 | 7 | 6 | 8 | 84–89 |
Behavioural tests (order of the tests) | Prepulse inhibition | 13 | 9 | 8 | 8 | 98–105 |
Elevated plus maze | 13 | 9 | 8 | 8 | 112–119 | |
Open field test and amphetamine-induced locomotor activity | 13 | 9 | 8 | 8 | 119–126 | |
Physiological test | Body mass | 13 | 9 | 8 | 8 | 133 |
Neurochemical test | Western blot | 13 | 9 | 8 | 8 | 133 |
Abbreviation: MRS, magnetic resonance spectroscopy; n6-POL, prenatal PolyI:C-exposed offspring treated with n-6 PUFA; n3-SAL, prenatal saline-exposed offspring treated with n-3 PUFA; n6-SAL, prenatal saline-exposed offspring treated with n6-polyunsaturated fatty acids (n-6 PUFA) control diet; PUFA, polyunsaturated fatty acids; Sal, saline.