Figure 2.

Genetic interactions between flamenco permissive alleles and gypsy insertions induce specific phenotypes. (A–E) Representative pictures of egg chambers subjected to β-galactosidase staining as readout for gypsy-lacZ reporter activity. (A) gypsy-lacZ reporter activity is repressed when the flam^su(f) restrictive allele is combined with the Df(1)l11 deficiency encompassing the flamenco locus. (B) When the same deficiency is combined with the X chromosome currying the flam^A allele, gypsy-lacZ reporter activity is strongly induced. (C) gypsy-lacZ reporter activity is repressed when the flam^FM7c restrictive allele is combined with the flam^A allele. (D) gypsy-lacZ reporter activity is induced in flam^A/flam¹ ovaries. (E) Combination of the Df(1)l11 deficiency with the ct^A allele in the flam^A-containing chromosome significantly reduces β-galactosidase staining. (F) Mortality during adult eclosion of ct^A flam^BG flies compared to the flam^BG strain. (G) Mortality during adult eclosion of ct⁶ and ctⁿ flies in their original genetic background and in combination with flam¹. (H) Mortality during adult eclosion of flam^A/flam¹ and ct^A flam^A/ct⁶ flam¹ female (F) flies (** P < 0.01). (I, J) Wings derived from male flies hemizygous for Bx² allele in (I) its original genetic background and (J) in flam¹ background. (K) Frequency of wing-blister phenotype of Bx² wings in their original genetic background and in combination with flam¹.