The gypsy insertion in the cut locus induces changes in the expression of gypsy and flamenco. (A) Map of the flamenco cluster showing the DIP1 gene and the flamBG02658 (flamBG) insertion at the 5′ end of the locus, the gypsy transposon fragments (thick bars), the positions of primers used to analyze flamenco primary transcripts expression (numbers 1–6), and the position of tested piRNAs (letters A and B). (B–E) qRT-PCR analysis of gypsy, ZAM, Idefix, and specific flamenco regions in RNA isolated from (B) 48-hr-old pupae, (C) 96-hr-old pupae, (D) 0- to 24-hr-old female adult heads, and (E) 3-day-old adult ovaries. Shown are average levels (n = 3), and error bars indicate SD (* P < 0.05, ** P < 0.01, *** P < 0.005).