Figure 5.

Cosuppression of gypsy and flamenco sequences from which small RNAs against gypsy originate. qRT-PCR analysis. Shown are the transcript levels of gypsy and two gypsy fragments in the flamenco locus (primers 5–6, described in Figure 3A) in RNA isolated from 48-hr-old pupae carrying the homozygous (A) ct^A or (B) f^A mutation induced by gypsy. (C) Expression of gypsy and gypsy fragments inside flamenco in 0- to 24-hr-old adult heads from flies carrying the ct^A homozygous mutation. (D) Expression of the distal gypsy fragment (primers 6) in w flam^A adult ovaries compared to that in 48-hr-old pupae. (E) piRNA levels in 3-day-old adult ovary detected by TaqMan specific probes (see Materials and Methods for details). Position of analyzed piRNAs loci are indicated in Figure 3A. Shown are average levels (n = 3), and error bars indicate SD (* P < 0.05, ** P < 0.01, *** P < 0.005).