Figure 6.

Both Rps2–GFP and Rpl25–GFP accumulate in the smc5-6 mutant. (A and B) Both the 40S and 60S subunits of ribosomal protein reporters accumulate in the smc5-6 mutant but not the smc6-9 mutant. Strains were transformed with either the Rps2–GFP or Rpl25–GFP reporter plasmid. Images were taken as described in Figure 3E. For either Rps2–GFP (A) or Rpl25–GFP (B) quantification, the strains carrying either Rps2–GFP or Rpl25–GFP were grown to midlog phase at 28° in dropout medium supplemented with 0.02 mg/ml adenine. Mean peak Rps2–GFP (A) or Rpl25–GFP (B) fluorescence intensity was measured by performing cytometry analysis as described in Figure 3E. P-values were measured using a t-test.