Figure 7.

Effect of PHO84 overexpression on the RLS of the rho⁺ strain. (A) The effect of an additional copy of the gene on PHO84 mRNA levels (relative to ACT1 mRNA) determined by RT-qPCR. The rho⁺ 2xPHO84 strain possessed a second copy of the gene at the ura3 locus (rho⁺ ura3Δ::PHO84). (B) The effect of an additional copy of PHO84 in the rho⁺ strain YPK9 (rho⁺ ura3Δ::PHO84) compared to both the rho⁺ (YPK9) and its rho⁰ derivative strain (YJR2). Mean RLS are 18.8, 21.4, and 25.6 for rho⁺, rho⁺ ura3Δ::PHO84, and rho⁰ strains, respectively. Lifespans for the first and second strain and for the first and third strain are significantly different (P = 0.014 and P < 0.001, respectively). They also differ significantly for the second and third strain (P = 0.021).