Figure 6.

Increased levels of 5MC and 5HMC on Gad1, Reln and Bdnf-ix promoter regions in the frontal cortex of PRS mice are reduced by treatment with valproate (VPA) Seventy-five day old mice were treated subcutaneously twice a day for 5 days with vehicle or 70 mg kg⁻¹ valproate. 5MC and 5HMC enrichment was measured on the sixth day, 20 h after the last treatment. No behavioral testing in these animals. Data are expressed as mean±s.e.m. and analyzed with one-way ANOVA followed by Student–Newman–Keuls multiple comparisons. *P<0.05 when vehicle (VEH)-treated PRS samples are compared with PRS VPA-treated samples or with treated or untreated NS samples. ANOVA, analysis of variance; BDNF, brain-derived neurotrophic factor; 5HMC, 5-hydroxymethylcytosine; 5MC, 5-methylcytosine; NS, nonstressed; PRS, prenatally stressed.