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. 2016 Jan 5;6(1):e703. doi: 10.1038/tp.2015.203

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Copyright © 2016 Macmillan Publishers Limited

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Generation of GAD67-tdTomato knock-in human iPSC lines. (a) Schematics of wild-type, donor and recombinant alleles of GAD67. P1–P4 indicate primer pairs 1–4. (b) Representative PCR analysis of puromycin-resistant clones by P1. Negative control indicates the PCR band from td^−/− iPSCs. (c) PCR analysis of puromycin-resistant clones using P2–P4. (d) GABA immunostaining reveals strong co-localization of tdTomato and GABA. Scale bar, 100 μm.