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. Author manuscript; available in PMC: 2016 Oct 18.
Published in final edited form as: Cell Rep. 2016 May 26;15(10):2214–2225. doi: 10.1016/j.celrep.2016.05.006

Figure 5. Improvement in Glucose Homeostasis by Hepatic DACH1 Silencing is Abrogated by Also Silencing ATF6.

Figure 5

(A–C) WT DIO mice were treated with AAV8–sh-DACH1 or AAV8–con. After 3 days, half of the AAV8–sh-DACH1 mice received adeno–sh-Atf6, while the other half received adeno-LacZ. After an additional 5 days, fasting blood glucose and plasma insulin were assayed, and glucose and insulin tolerance tests were conducted (n = 6 mice/group; mean ± SEM, p< 0.05).

(D) WT DIO mice were treated as in (A–C). After 14 days, the mice were fasted for 5 h and injected with insulin through the portal vein, followed 3 mins later by harvesting of liver. Total liver extracts were assayed for p-Akt, total Akt, and β-actin (top blot), and nuclear extracts were assayed for ATF6 and nucleophosmin (Np) (bottom blot).