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. 2016 Aug 15;6(10):3017–3026. doi: 10.1534/g3.116.031229

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Copyright © 2016 Kockel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunohistochemical analysis of StanEx enhancer trap expression in third instar larval tissue visualized by LexAop-CD8::GFP. (A) w; StanEx^DT-2/LexAop-CD8::GFP. Expression in fat body. Note the variable expression in fat body cells. (B) w; StanEx^EH-2/LexAop-CD8::GFP. Expression in anterior midgut. Note that expression in garland nephrocytes is lexAop-CD8:GFP background signal (see Materials and Methods and Figure S6). (C) w; StanEx^SX-4/+; LexAop-CD8::GFP/+. Expression in midgut. (D) w; StanEx^LH-3/+; LexAop-CD8::GFP/+. Expression in malphigian tubules. Note the variable expression in individual cells. (E) w; StanEx^HS-2/LexAop-CD8::GFP. Expression in trachea located on midgut. (F) w; StanEx^LH-4/LexAop-CD8::GFP. Expression in small cells in midgut consistent with expression patterns of entero-endocrine cells. (G) w; StanEx^JHW-2/LexAop-CD8::GFP. Expression in photoreceptor clusters in third instar eye disc. (H) w; StanEx^IP-1/+; LexAop-CD8::GFP/+. Expression in third instar haltere, leg, and wing disc. Green, Anti-GFP; Red, Anti-Tubulin; Blue, DAPI. Scale bar = 50 μm.