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. 2016 Aug 2;6(10):3139–3148. doi: 10.1534/g3.116.032300

Figure 5.

Figure 5

Schematic of an in vivo assay for transvection activity based upon interallelic transcriptional enhancement. All transgenes are inserted at the attP2 locus and the size of the arrow represents the rate of transcription at the associated locus. (A) Hemizygous UAS-PrP is expressed at a low level. (B) Homozygous UAS-PrP is expressed at a high level due to the in trans interaction between UAS enhancers at the same locus. (C) UAS-PrP in combination with UAS-shRNAs targeting genes lacking transvection activity (irrelevant shRNA) also leads to high level PrP expression due to the preserved interaction between UAS enhancers in trans. (D) UAS-PrP in combination with UAS-shRNAs targeting genes with transvection activity (relevant shRNA) interfere with the trans interaction between UAS enhancers, reducing PrP expression. The degree to which target gene knockdown impacts transvection can be simply quantified in this system by a Western blot of PrP levels. PrP, prion protein; shRNA, short hairpin RNA.