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. 2016 Aug 2;6(10):3139–3148. doi: 10.1534/g3.116.032300

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Copyright © 2016 Noble et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Expression of shRNAs against two putative chromosomal pairing factors, pbl and aurB, reduces interallelic transcriptional enhancement in vivo. Flies expressing UAS-PrP in trans to UAS-shRNAs directed against 16 different genes thought to be involved in chromosomal pairing were generated. Transgene expression was driven in cholinergic neurons by Cha-GAL4 and interallelic enhancement of PrP expression was measured by Western blot of fly head homogenates. PrP expression levels were normalized to the level seen in the presence of an anti-GFP control hairpin in trans. Error bars represent standard deviations, with n = 3 for experimental samples and n = 4 for control samples; **** P < 0.0001. We found that shRNAs against pbl and aurB significantly decreased interallelic enhancement of PrP expression. GFP, green fluorescent protein; PrP, prion protein; shRNA, short hairpin RNA.