Table 1. Testing viability of chromosomal pairing factor knockdown in vivo.
| Adult Viability with shRNA Expression | |||
|---|---|---|---|
| Gene | BDSC shRNA Stock Number | Cha-GAL4 > shRNA | α-tubulin-GAL4 > shRNA |
| CG2469 | 33,736 | − | N/a |
| chb | 34,669 | − | N/a |
| Dhc64C | 36,698 | − | N/a |
| Klp61f | 33,685 | − | N/a |
| Nlp | 33,688 | − | N/a |
| shtd | 38,531 | − | N/a |
| SkpA | 32,991 | − | N/a |
| slmb | 33,986 | − | N/a |
| Tlk | 33,983 | − | N/a |
| Arp1 | 32,032 | + | − |
| aurB | 28,691 | + | − |
| cal1 | 41,716 | + | − |
| CG7236 | 27,505 | + | − |
| CKIα | 25,786 | + | − |
| Cul1 | 29,520 | + | − |
| Det | 51,837 | + | − |
| E(bx) | 33,658 | + | − |
| fzy | 40,933 | + | − |
| Hsc70Cb | 33,742 | + | + |
| ida | 34,552 | + | − |
| Lis-1 | 35,043 | + | − |
| Nc73EF | 33,686 | + | − |
| pbl | 28,343 | + | − |
| polo | 33,042 | + | − |
| su(Hw) | 34,006 | + | + |
| w | 33,623 | + | + |
shRNAs from the TRiP library against the listed genes, which have been identified in previous studies as playing a role in somatic homolog pairing, were obtained from the BDSC. shRNA expression was driven in cholinergic neurons with the Cha-GAL4 driver, or ubiquitously with the α-tubulin-GAL4 driver. shRNA against the w gene was included as a positive control. shRNA, short hairpin RNA; BDSC, Bloomington Drosophila Stock Center; −, no viable adult flies recovered; N/a, cross not performed; +, viable adult flies recovered; TRiP, Transgenic RNA Interference Project.