Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Aug 8;6(10):3161–3168. doi: 10.1534/g3.116.032482

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016 Zebec et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

(A) Schematic representation of programmed silencing of the α-amylase of S. solfataricus in a transfection assay using a multiplex miniCRISPR locus (miniCR-AA12345). Locations of primers used in qPCR are indicated (Q-AA2-sp-FW and Q-AA2-sp-RV). (B) Protospacer (i.e., targeting sites) regions in α-amylase mRNA with 3′ PAS hybridizing to matching crRNA and 5′ handle. Exact protospacer positions on mRNA sequence are indicated. Upper case letters, complementarity; lower case letters, mismatch; linked lower case letters, G:U pairing through mismatch.