Figure 5.

Conversion tracts in armi recombinants. The homologous donor carried part of the armi gene, a Strep-tag II peptide tag at the end of coding sequence, and 18 sites (inverted triangles) differing in sequence from the endogenous locus that allowed mapping of conversion tracts. Dots on the donor plasmid mark silent mutations that confer resistance to the armi sgRNAs. Closed circle, site converted to the donor sequence. Each line presents one recombinant, and the color of closed circles corresponds to the color of the DSB(s) from which HR was initiated (dotted vertical lines). An × indicates an indel.