Figure 7.

Lig4¹⁶⁹ mutant does not inhibit end joining or improve HR. Adults from injected G0 embryos that produce G1 broods were divided into three groups according to their eye color composition: (1) all red; (2) having at least one white, but no coffee, G1; and (3) having at least one coffee G1. For each w sgRNA, the percentage of coffee G1 in individual group 3 broods was compared between Lig4⁺ or Lig4¹⁶⁹ embryos. Similarly, the percentage of white G1 in broods with at least one white G1 (with or without coffee G1) was compared. All datasets failed the Shapiro–Wilk normality test, and therefore the two-tailed Mann–Whitney Rank Sum test was used to calculate the p-value. NA, N was too small to compute a p-value.