Figure 2.

Protein expression and location of wild-type (WT) and mutant DNAAF1. (A) Expression of GFP-tagged DNAAF1 protein is shown by a Western blot analysis of whole cell lysate with anti-DNAAF1. α-tubulin was used as a loading control. Protein expression level of DNAAF1 c.691A > C was almost identical with the wild type, while DNAAF1 c.1022_1023delAA could hardly be detected. (B) Immunofluorescence staining showing protein location. Both c.691A > C mutant and wild-type GFP-tagged DNAAF1 protein localized in the cytoplasm, whereas no green immunofluorescence was detected in c.1022_1023delAA mutant transfected HEK293T cells. Transfected cells are marked by GFP (green).