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. 2016 Aug 18;6(10):3307–3316. doi: 10.1534/g3.116.033696

Figure 3.

Figure 3

Axonemal defects of the IDA (DNALI1) in MDCK cells transfected with DNAAF1 missense mutant. High-resolution immunofluorescence microscopy of MDCK cells was performed with antibodies directed against the IDA component DNALI1 (A) and ODA chains DNAI2 (B). In control and GFP-tagged DNAAF1 wild-type (WT) transfected cells, DNALI1 and DNAI2 localize within the cytoplasm, whereas DNALI1 labeling is absent from the DNAAF1 p.Lys231Gln mutant transfected MDCK cells. The staining pattern of DNAI2 (B) is not obviously changed for the mutant. Nuclei were stained with DAPI (blue). GFP and GFP-tagged DNAAF1 protein are shown in green. DNALI1 and DNAI2 are shown in red.