Figure 1.

Primary screen. (A) All 3400 gene deletion strains from the Bioneer collection were spotted to YES-agar plates containing DMSO (solvent control) or 0.4 μM LatA and then incubated for 3 d at 30°. Two representative plates from the screen are shown. A wild-type strain, as well as two hyper-sensitive mutants, lsk1Δ and pap1Δ, were used as controls (green circles). Two “hits,” rpl2102 and mcl1Δ, are also highlighted (red circles). (B) Venn diagram analysis of the three trials of the primary screen. Thirty-eight hits were common to all three trials and comprise the high-confidence group (red). Seventy-seven hits were common to two of the trials and comprise the medium-confidence group (green). One hundred and seventy-three hits were common to one of the three trials and comprise the low-confidence group (blue).