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The gene deletion mutants of the high-confidence list are prone to cytokinesis failure in the presence of LatA. Cells of the indicated genotype were grown to midlog phase and then treated with DMSO (solvent control) or 0.3 μM LatA for 5 hr. Cells were fixed and stained with DAPI/aniline blue to visualize nuclei and cell/wall septal material, respectively. Four strains from the high-confidence list are shown as representative examples.
