Figure 1.

Identification of the ADF1 gene. (A). A schematic depicting gene predictions within the genomic region known to contain the ADF1 gene on the basis of recombination mapping. Shown below are the boundaries of BACs spanning this region. (B) A schematic of the genomic region covered by BAC 24I14, which complements the adf1 mutation, showing gene predictions and EcoRI restriction sites (black lines). (C). Deflagellation responses of WT, adf1-3, and the strain of adf1-3 rescued with EcoRI-digested BAC 24I14 (adf1-3R). Mean of three independent experiments ± SD.